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Gel-to-gel correlation of five replicate gels showing 2-DE reproducibility. Log normalized spot volumes for gel 1 were plotted pairwise versus gels 2 to 5 and the Pearson’s correlation coefficient shown in squares was calculated. Gels (11 cm) were focused over the pH range 3–10 and were stained with Sypro Ruby. Gel image analysis utilized Nonlinear SameSpots software.
