Table 2.

Summary prefractionation protein ID’s

Fractions analyzed	Protein IDs	Non-redundant Protein IDsa
Subcellular fractionsb
1-cytoplasm	625	95
2-organelle	425	50
3-nucleus	693	141
4-cytoskeleton	345	52
Total subcellular	2088	338
IEF fractionsc
pH 3.0–5.4	44	4
pH 5.4–7.0	181	13
pH 7.0–10.0	226	16
Total IEF	451	33
Whole cell lysate	480	63
Total all fractions	3019	434

^aHad Mascot expectation scores of 10⁻³ or less.

^bAccording to Calbiochem’s ProteoExtract® Subcellular Proteome Extraction kit.

^cUsing ZOOM^® IEF Fractionator (Invitrogen).