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. 2010 Sep 21;103(8):1284–1291. doi: 10.1038/sj.bjc.6605884

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Copyright © 2010 Cancer Research UK

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Activation of the ERBB2/ERBB3 signalling pathway by BCAR4. (A) Lysates of ZR-75-1 cells transduced with empty vector, BCAR4, BCAR3, AKT1 or AKT2 were immunoprecipitated with phosphotyrosine-specific antibody and subjected to western blot analysis. An approximately 180-kD band in lysates from ZR/BCAR4 cells is marked with an arrow. To identify the phosphorylated proteins in this band, identically loaded blots were probed with antibodies against EGFR, ERBB2, ERBB3 and ERBB4. Phospho-EGFR was not detected (data not shown). (B) Activation of downstream signalling of ERBB2/ERBB3. Lysates of two independent pools of ZR-75-1 cells containing empty vector or stably expressing BCAR4, cultured in the presence of oestradiol (E) or 4-OHT (T), were subjected to western blot analysis. Blots were probed with total ERBB2, ERBB3, phospho-AKT, total-AKT, phospho-extracellular signal-regulated kinase (ERK)1/2 and total-ERK1/2 antibodies and β-actin for loading control.