. 2001 Jan 15;29(2):582–588. doi: 10.1093/nar/29.2.582

Table 1. Summary of sequencing data.

	Number	Match length (bases)			Ambiguity (%)
		Average ± SD	Range	Median	Average ± SD	Range	Median
Stocks received	1189	n/a	n/a	n/a	n/a	n/a	n/a
Failed to grow	56	n/a	n/a	n/a	n/a	n/a	n/a
No sequence read	10	n/a	n/a	n/a	n/a	n/a	n/a
Correct (pre-screened)^a	613	383 ± 73	54–549	399	2.0 ± 2.5	0–17.0	1.2
Correct (post-screen)	126	363 ± 96	94–500	389	2.0 ± 2.0	0–11.3	1.4
Correct (isolated from contaminated stocks)	84	372 ± 79	75–467	396	1.6 ± 2.1	0–14.3	1.2
Incorrect (pre-screened)	152
Accession no. in UniGene cluster	97	386 ± 56	186–490	399	2.4 ± 2.2	0–9.3	1.7
Accession no. not in UniGene cluster	15	380 ± 100	182–476	427	1.7 ± 2.1	0.5–8.6	0.9
No significant identity	40	n/d	n/d	n/d	n/d	n/d	n/d
Incorrect (colony isolation)^b	332
Accession no. in UniGene cluster	247	369 ± 76	111–525	383	1.7 ± 1.6	0–9.0	1.2
Accession no. not in UniGene cluster	61	395 ± 85	149–546	424	1.4 ± 1.6	0–7.0	0.8
No significant identity	24	n/d	n/d	n/d	n/d	n/d	n/d
Total clones identified^c	1243	378 ± 76	54–549	394	1.9 ± 2.2	0–17.0	1.2
Total clones^d	1303	n/a	n/a	n/a	n/a	n/a	n/a

From 1189 stock cultures ordered, the number of entities in each category is noted. Correct is defined as sequence that has identity to the published sequence for the desired clone. Incorrect sequences do not have identity to the desired clone. Incorrect sequences were assigned GenBank accession numbers where possible by comparison to the murine sequences in dbEST. Match length (the length of the region of identity between derived and published sequence) is presented with standard deviation, range and median values. Percent ambiguity (the number of ambiguous base calls divided by the total number of bases over the region of identity) is also presented with standard deviation, range and median values. n/a, Not applicable; n/d, not determined.

^aPre-screened, derived from those stocks which passed agarose gel electrophoretic pre-screening.

^bColony isolation, derived from those stocks which failed pre-screening.

^cIncludes correct clones, and incorrect clones which could be assigned a GenBank accession number cluster. This number is higher than the number of stocks ordered, due to the isolation of more than one incorrect clone from several stock cultures.

^dNumber of clones examined, inclusive of clones which could not be identified.