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Formation of the oxidized nucleotides and nucleosides by ROSs. The nucleotides and nucleosides were treated with Fe(II)–EDTA and were analyzed by HPLC as described in the Materials and Methods. The production of (A) 2-OH-rATP from ATP, (B) 2-OH-rAMP from AMP, (C) 2-OH-rAdo from Ado, (D) 8-OH-rGTP from GTP, (E) 8-OH-rGMP from GMP and (F) 8-OH-rGuo from Guo are shown.
