Table 3.

Distribution of p57Kip2 and p27Kip1 positive cells in the proliferative (Prolif) and non-proliferative (Non-P) regions of the E14.5 diencephalon.

Structure	Region	p57+ Cell Distribution (%)	p27+ Cell Distribution (%)	p27+ p57+ per total p57+ Cell
Epithalamus (2482*)	Prolif (P)	73.8 ± 9.5	20.7 ± 3.2	34.3 ± 2.1
	Non-P	26.2 ± 4.7	79.3 ± 6.7	48.5 ± 4.6
Dorsal Thalamus (3874)	Prolif	4.4 ± 2.0	32.3 ± 2.5	12.2 ± 1.8
	Non-P	95.6 ± 2.0	67.7 ± 2.5	73.0 ± 2.6
Hypothalamus (3330)	Prolif	92.2 ± 1.1	21.0 ± 1.8	68.1 ± 4.6
	Non-P	7.8 ± 1.1	79.1 ± 4.6	7.6 ± 1.0

E14.5 embryos were processed for combined p57Kip2 and p27Kip1 immunolabeling and DAPI nuclear staining. At least 2 sections from each of 2–3 embryos per region were assessed using confocal imaging at 400X magnification for the distribution of positive cells between the proliferative and non-proliferative regions in each structure. The percent of p57Kip2-expressing cells that also co-labeled for p27Kip1 was calculated as a ratio to total p57Kip2 cells. Data are expressed as mean ± SD.

^*indicates the total number of cells counted for each structure.