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. 2010 Oct;131(2):174–182. doi: 10.1111/j.1365-2567.2010.03286.x

Figure 4.

Figure 4

Small interfering RNA (siRNA) against proviral integration site kinase 2 (PIM2) knocked down interleukin-6 (IL-6) expression. HeLa cells were transiently transfected with PIM2-specific and control siRNA for 24 hr. Cell pellets were harvested after the cells were stimulated with tumour necrosis factor-α (TNF-α; 10 ng/ml) for 4 hr. Real-time reverse transcription–polymerase chain reaction was performed to detect the messenger RNA (mRNA) level of PIM2 and IL-6. The culture supernatants were harvested after the cells were stimulated with TNF-α (10 ng/ml) for 18 hr. The IL-6 protein concentration was detected by enzyme-linked immunosorbent assay. Data are representative of three separate experiments. †P < 0·05; *P < 0·01 versus PIM2 siRNA-treated group. For the PIM2 mRNA group, the comparison is between control siRNA and PIM2 siRNA treated with and without TNF-α. For IL-6 mRNA and protein groups, the comparison is between samples treated with TNF-α only.