Figure 5.

Proviral integration site kinase 2 (PIM2) small interfering RNA (siRNA) knocked down nuclear factor-κB (NF-κB) luciferase activity. HeLa cells stably transfected with the vector containing NF-κB promoter and luciferase reporter were transiently transfected with PIM2-specific and control siRNA for 24 hr. The NF-κB activity was determined by its luciferase expression after cells were stimulated with tumour necrosis factor-α (TNFα) (a) or interleukin-1β (IL-1β) (b) for 24 hr. Data are representative of two separate experiments. †P < 0·05; *P < 0·01 compare the average of Ctrl-1 and Ctrl-2 siRNA-treated groups with the average of PIM2-1 and PIM2-2 siRNA-treated groups.