Table 2.

Puc18 sequence

	Puc18 sequence
1	tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61	cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121	ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181	accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241	attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301	tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361	tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
421	actctagagg atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct
481	gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
541	aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
601	gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
661	agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
721	gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca

Forward primer: cggcatcaga gcagattgta; reverse primer: gg cggtaatacg gttatcca; EcoR1 Site: g aattcg.