Table 3. Interaction between α proteins detected by the yeast two-hybrid system.

Plasmidsa	Protein fused tob		Activation of	Activation of lexAop8-lacZ
	LexA DNA-binding domainc	B42 activation domain	lexAop6-LEU2d (Eop)	Activitye (U β-galactosidase)	Colorf
pEG202 pJG4–5	–	–	<1 × 10–3	3.16 ± 0.68	White
pEG202 pGM591	–	α	<3 × 10–3	3.46 ± 0.10	White
pGM585 pJG4–5	α	–	<5 × 10–3	1.02 ± 1.12	White
pGM585 pGM591	α	α	1	350.30 ± 29.20	Dark blue
pGM588 pGM607	αcrG732W	αcrG732W	1	Not tested	Dark blue
pGM585 pGM607	α	αcrG732W	1	Not tested	Dark blue
pGM588 pGM591	αcrG732W	α	1	Not tested	Dark blue

^a–eSee Table 2.

^fColor of colonies grown in glucose medium in the presence of tryptophan, leucine and X-Gal.