oriC227 suppresses the over-initiation phenotype of seqAΔ.
A. Growth rates of wt or seqAΔ cells carrying the indicated oriC allele. Averages of two independent experiments (± SD) shown.
B. Nucleoid morphology and DNA content were assayed in seqAΔ cells carrying the indicated oriC alleles. DNA histograms were generated from rifampicin run-off cultures. Unresolved peaks in seqAΔ oriC+ cells indicate an inability to complete replication elongation. All cells were grown in M9 glucose plus casamino acids.