Skip to main content
. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: J Cell Biochem. 2010 Aug 1;110(5):1073–1081. doi: 10.1002/jcb.22619

Figure 2. Effect of hyperthermia in combination with TRAIL on cell viability.

Figure 2

CX-1 tumor cells were exposed to hyperthermic (42°C) or normothermic (37°C) conditions for 1 h in conjunction with and without 10 ng/ml of TRAIL. After treatment, cells were then exposed to 37°C for 3 h in the presence of 10 ng/ml of TRAIL. After 3 h exposure, cell survival was determined by colony formation. For colony formation, cells were trypsinized, counted, and plated. After 10 days, colonies were counted and survival was determined as described in Materials and Methods. Error bars represent standard error from the mean (S.E.M.) for three separate experiments. Asterisk * represents a statistically significant difference between control (37°C only) and heat (42°C only) or heat + TRAIL-treated cells at P<0.01.