Fig. 5.

S100A5 expression was detected in a subset of OSNs in a mosaic pattern. A,A′: Although expression of S100A5 occurs throughout the olfactory epithelium, OSNs that appear to lack expression of S100A5 can be seen as gaps in the labeling in the OSN layer. B: These gaps are easier to see near the lateral margins of the epithelium, where OSNs are less dense. C: Dual FISH confocal image for S100A5 (red) and OMP (green) detects OSNs expressing OMP that appear to lack S100A5. C′: Letting a similar image oversaturate to detect overlapping expression more rigorously continues to indicate OSNs lacking detectable S100A5 expression (arrows). D: Cells expressing S100A5 (red) do not also express GAP-43 (green). Apical is upward. Scale bars = 25 μm in A–D; 250 μm in A′.