Figure 7. The effect of MITF knock-down on TRPM1 and miR-211 expression in pigmented melanoma cells; SKMEL-28.

Relative gene expression levels of MITF, TRPM1, and miR-211 in MITF knock-down cells. Three different doses of MITF siRNA (5 nM, 10 nM and 15 nM) was used to knock-down MITF gene and expression values are normalized to scramble siRNA control. Histograms represent the Ratio of RNA Concentration, as measured by qRT-PCR analysis.