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. 2010 Nov 1;5(11):e13780. doi: 10.1371/journal.pone.0013780

Figure 5. Expression of cell cycle-regulatory genes in MS4a4B-LV-infected EL4 cells.

Figure 5

A, Total RNA was isolated from MS4a4B-LV vector- or mock LV vector-infected EL4 cells. Expression of 84 key regulatory genes on cell cycle progression was determined with real time PCR array analysis kit (Cat#: PAMM-020, SABiosciences, Gaithersburg, MD). Data were analyzed with on-line analysis software according to the manufacturer's instructions and presented as scatter plot with fold change of genes. Red dot: genes increased by ≥4 fold; blue dot: genes decreased by ≥4 fold. B, Genes with fold change ≥4 were shown as column figure. C, MS4a4B (or control) vector-infected EL4 cells were synchronized and stimulated with 15% FCS for 8 hours. Cell lysates (100 μg) were immunoprecipitated with anti-Cdk2-protein A beads. Cdk2-cyclin activity was assayed as described in “Methods”. The same samples (25 μg) were used for western blot with anti-β-actin antibody to ensure identical loading. Relative intensity (sample vs. LV control) is shown in the lower panel. *, p<0.05. D, Cell lysates described in “C” were separated on 10% SDS gel followed by western blotting with appropriate antibodies as indicated. E. Relative intensity of blots in “D” (sample vs. internal control (β-actin)) was determined by densitometry. *, p<0.05.