Skip to main content
. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: J Neurochem. 2008 Sep 20;107(3):734–744. doi: 10.1111/j.1471-4159.2008.05644.x

Figure 2. Single strand breaks-processing in brain and mucosal nuclear extracts.

Figure 2

Reactions were assembled with radioactively labeled substrates (Table 1) and purified proteins or nuclear extracts as indicated. Products were resolved in denaturing polyacrylamide gels and visualized by autoradiography; representative autoradiograms are shown. Time points are indicated, and in each case, a substrate/BSA lane was included; mixtures of labeled oligonucleotides were resolved in parallel to provide length markers (M). A) Reactions assembled with purified proteins yielded a 45-mer product. B) To remain in linear range, different time points were used with mucosal (4, 8, 16 min) and brain (8, 16, 32 min) extracts. With mucosal extracts, a significant amount of product was seen already by 8 minutes, with all substrate consumed by 32 minutes (lane 24). C) Time courses: product yields were calculated from phosphorimager values using the ImageQuant software. Values for 3–4 independent assays for each substrate in the linear range of reaction were used to obtain means and SEM and plotted as a function of time. Yields for brain extracts are coded in red.