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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: J Neurochem. 2008 Sep 20;107(3):734–744. doi: 10.1111/j.1471-4159.2008.05644.x

Figure 4. Processing of gapped DNA with a 5′-THF blocked terminus in assays reconstituted with purified proteins.

Figure 4

A) The 5′-labeled gap-[5′-THF] duplex oligonucleotide substrate was assembled with indicated combinations of purified proteins. The different synthesis and ligation products are demarcated. FEN1 was required for efficient pol β-catalyzed longer than [+1] synthesis and efficient generation of the 45-mer in the presence of ligase l or lll. Labeled oligonucleotides provide size references (M). B) Pol β catalyzed gap filling synthesis on a 9-nt gap substrate (Table 1) was used to demonstrate an effect of PCNA when used on linear duplex oligonucleotide substrates.