Table 2.
Production of chemokines by lung lymphocytes ex vivo after immunization with Ad85A i.d. or i.n..
| Absorbance at 450nm (± SD) | ||
|---|---|---|
| Chemokine | i.d. | i.n. |
| CCL2 | 0.89 (± 0.427) | 1.368 (± 0.131) |
| CCL3 | 0.334 (± 0.066) | 0.745 (± 0.008) |
| CCL4 | 0.278 (± 0.011) | 0.760 (± 0.028) |
| CCL5 | 0.386 (± 0.098) | 1.470 (± 0.211) |
| CXCL9 | 0.259 (± 0.059) | 0.566 (± 0.034) |
| Conc (pg/ml) | ||
| CXCL16 | 3.34 (± 0.339) | 6.095 (± 0.977) |
Lung lymphocytes were isolated from mice immunized 3 weeks previously and cultured in RPMI+10% FCS for 6 hours at 37°C without antigen stimulation. The supernatants were assayed by ELISA for the indicated chemokines. The table shows the mean absorbance at 450 nm (± standard deviation (SD)) of 6 mice per group. For CXCL16, the mean concentration of CXCL16 (pg/ml, ± SD) of 6 mice per group is shown.