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. 2001 Jan 15;29(2):423–429. doi: 10.1093/nar/29.2.423

Figure 1.

Figure 1

TTF-I stimulates transcription on terminator-containing templates. (A) Schematic representation of the templates used. The open bar marks 5′-terminal murine rDNA sequences, the black line 3′-terminal rDNA sequences and the black boxes indicate the position of the upstream and downstream terminator elements T0 and T2, respectively. Run-off and terminated transcripts are indicated by a dashed arrow. (B) Transcription assays. Reactions contained 20 ng of the indicated template DNA, 6 µl S-100 extract and either no TTF-I (lanes 1, 4 and 8), 5 fmol (lanes 2, 5 and 9), 10 fmol (lanes 6 and 10) or 20 fmol (lanes 3, 7 and 11) of recombinant TTFΔN185.