TTF-I stimulates transcription
on terminator-containing templates. (A) Schematic
representation of the templates used. The open bar marks 5′-terminal murine
rDNA sequences, the black line 3′-terminal
rDNA sequences and the black boxes indicate the position of the
upstream and downstream terminator elements T0 and T2,
respectively. Run-off and terminated transcripts are indicated by
a dashed arrow. (B) Transcription assays. Reactions
contained 20 ng of the indicated template DNA, 6 µl
S-100 extract and either no TTF-I (lanes 1, 4 and 8), 5 fmol (lanes
2, 5 and 9), 10 fmol (lanes 6 and 10) or 20 fmol (lanes 3, 7 and
11) of recombinant TTFΔN185.