Figure 7.

Effect of CCl₄ with or without prior administration of natansnin on Caspase-3 and cytochrome-C. Whole cell protein extracted from control, CCl₄ and natansnin (10 mg/kg, 20 mg/kg body weight) treated rats and separated on 12% SDS gels and transferred on to nitrocellulose membranes. Immunoblots were detected with Caspase-3 and cytochrome-C specific primary antibodies. The expression levels of β-actin were used as loading controls. Densitometric analysis showing the protein levels of Caspase-3 and cytochrome-C were increased in CCl₄ group while treatment with natansnin significantly decreased the protein levels. Data are represented as mean ± standard deviation from three independent experiments (*p < 0.05 indicate significant difference relative to the CCl₄ treated rats).