Figure 9.

Effect of CCl₄ with or without prior administration of natansnin on Bax and Bid. Mitochondrial protein extracted from control, CCl₄ and natansnin (10 mg/kg, 20 mg/kg body weight) treated rats and separated on 10% SDS gels and transferred on to nitrocellulose membranes. Immunoblots were detected with Bax and Bid specific primary antibodies. The expression levels of β-actin were used as loading controls. Densitometric analysis showing the protein levels of Bax and Bid were increased in CCl₄ group while treatment with natansnin significantly decreased the protein levels. Data are represented as mean ± standard deviation from three independent experiments (*p < 0.05 indicate significant difference relative to the CCl₄ treated rats).