Figure 4.

PIST reduced the membrane expression of cadherin 23 in transfected HEK293 cells. A. Myc-cadherin 23 immunoreactivity using a non-permeable protocol shows clear plasma membrane localization. B. In the presence of EGFP-PIST, Myc-cadherin 23's plasma membrane immunoreactivity is undetectable. Staining was performed as normal except Triton X-100 was excluded. Myc-cadherin 23 was stained with mouse anti-Myc antibody and then Cy5-conjugated goat anti-mouse secondary antibody. mCherry was used as a control indicating Myc-cadherin 23-IRES-mCherry cassette was expressed successfully. Scale bar, 10 μm.