Figure 2.

Time course of human peripheral blood lymphocytes (PBL) bound per high endothelial venule (HEV) of peripheral lymph nodes (PLN) (A), Peyer’s patches (PP), (B) or endothelial cells of small blood vessels of pancreas (C) over pregnancy and postpartum periods. Data shown are from one experiment using PBL from one anonymous human donor and the same mouse as donor for all tissues. Similar outcomes were obtained in replicate (n=3) experiments that examined the full gestational time-course with peak adherence detected between gd 6 and 8. Data are the mean number ± SD of lymphocytes bound/vessel and are based on triplicate counting of 300–500 HEV or small vessels. Function-blocking mAb specific for L-selectin (DREG-56) or MAdCAM-1 (MEA-367) significantly reduced adhesion compared with untreated controls (p<0.001 using Student’s t test). * Indicates a significantly different mean (p<0.05) to that in the same tissue from virgin females (V) as analyzed by 2 way ANOVA (SAS 6.12, SAS Institute, Cary, NC).