Figure 6.

CD56bright cells adhere to weakly expressing Tie2-GFP+ vascular endothelium in the central decidua basalis. Confocal images of 12 μm cryosections of uterine tissue from Tie2-GFP transgenic mice at gd 8 are presented. Human lymphocytes pre-labeled with anti-CD56-PE-Cy5 were applied to frozen tissue section under mechanical shear. In the left panel, vascular endothelium in the decidua parietalis, lateral to the central decidua basalis, strongly expresses GFP but does not mediate lymphocyte adhesion. In the right panel, vessels in the central decidua basalis (DB) demonstrate weaker expression of Tie2-GFP but support adhesion of CD56bright cells (indicated by arrow); arrowheads demark perimeter of vessels. Bar = 50 μm in each image.