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. 2010 Oct 19;10:94. doi: 10.1186/1471-2377-10-94

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Copyright ©2010 Horzinski et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of the lymphocytes EBV-transformation on the expression level of the four mRNA markers of the ER-stress response. Thapsigargin (incubation with Thapsigargin (Tg) at 2 μg/ml for 4 h) was used to induce ER-stress in lymphoblastoid cell lines (EIL, black bars) and primary lymphocytes (PL, white bars) from control (C1, C2 and 1283-1) and eIF2B-mutated (1241-1, 807-1, 648-1 and 648-2) patients. Expression levels of ATF4 (A), GRP78 (B), ASNS (C) and CHOP (D) mRNA were determined by quantitative real-time polymerase chain reaction. The level of each transcript was calculated using the 2^(-ΔΔCt) method, followed by normalization of the primary Ct data to beta2M level. Results are shown as fold-change between ER-stress (Tg incubation) compared to normal conditions.