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. 2010 Oct 13;9:271. doi: 10.1186/1476-4598-9-271

Figure 3.

Figure 3

Expression of serpinE2 in colorectal cancer cells is dependent on MEK/ERK activation. A- Total RNA was isolated from CRC cell lines and processed for Q-PCR to analyze serpinE2 gene expression as described in Material and Methods. The relative level of each RNA was calculated using the standard curve method and normalized to the corresponding β2MIC RNA level. *, significantly different from Caco-2/15 (set at 1) at p < 0.01 (Student's t test). B- HCT116 and LoVo cells were treated daily with U0126. After two days, cells were lysed and total RNA was isolated for Q-PCR for serpinE2 gene expression. The relative level of each RNA was calculated using the standard curve method and normalized to the corresponding β2MIC RNA level. *, significantly different from control (DMSO) at p < 0.05 (Student's t test).