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. 2010 Oct 18;9:286. doi: 10.1186/1475-2875-9-286

Figure 5.

Figure 5

Western blot analysis. A. SDS-PAGE gel of OFFGEL™ fractions probed with anti-DHFS-FPGS. B. SDS-PAGE gel of OFFGEL™ fractions probed with anti-GTPCI. Anti-PTPS was used as a positive control after blotting and probing for DHFS-FPGS. The isoelectric point and molecular mass of both DHFS-FPGS and GTPCI differ significantly from their theoretical values. DHFS-FPGS would be expected to be identified from lane 6 at molecular mass 60 kDa, yet western blotting analysis places it in lane 3. GTPCI has a theoretical mass of 46 kDa and would be expected to be identified from lane 12, yet this analysis identifies it as being found in lanes 9 and 10 and at mass 60 kDa