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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Acta Biomater. 2010 Aug 3;7(1):268–277. doi: 10.1016/j.actbio.2010.07.036

Fig. 6.

Fig. 6

Unstained low magnification TEM images showing the extent of collagen fibril mineralization within one square frame of a 400-mesh formvar- and carbon-coated grid containing reconstituted type I collagen fibrils. The grids were all retrieved from the mineralization medium after 24 h. The black periphery represents the grid bars. A. No mineralization (PVPA absent). B. Sparse mineralization (collagen cross-linked with EDC to immobilize 4,000 or 8,000 μg/mL PVPA). C. Moderate mineralization (collagen cross-linked with EDC to immobilize 200 or 2,000 μg/mL PVPA). D. Dense mineralization (collagen cross-linked with EDC to immobilize 500 or 1,000 μg/mL PVPA).