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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Gastroenterology. 2010 Jul 14;139(5):1630–1641.e2. doi: 10.1053/j.gastro.2010.07.006

Figure 1.

Figure 1

MDP stimulates autophagy in epithelial and dendritic cells. (A) Immunoblot of HCT116 cells treated with 10µg/mL MDP. LC3-II accumulation quantified by LC3-II/GAPDH ratio. p-p38, phosphorylated p38. (B) Assay performed as in A. with primary human dendritic cells. (C) Confocal micrographs of HCT116 expressing EGFP-LC3 (green) in media or MDP-stimulated (20µg/mL) for 1h. (D) Quantification of cells with punctuate EGFP-LC3, mean ±SD, n≥4 fields with >50 cells/field. (E) Electron micrograph of MDP-stimulated HCT116:LC3 cells (10µg/mL for 1h). Arrows indicate autophagosomes (F) Immunoblot of MDP-LL-stimulated (10µg/mL) HCT116 cells.

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