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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Gastroenterology. 2010 Aug 1;139(5):1730–1739.e1. doi: 10.1053/j.gastro.2010.07.046

Figure 2. IRE1 activation and expression of downstream targets in MCD-fed mice.

Figure 2

(A) IRE1 activation and XBP-1s expression were assessed by immunoblotting in liver homogenates from mice fed MCS (−) or MCD (+) formulas for 4–21 days. (B) IRE1 activation was compared in liver homogenates from mice fed chow, MCS, or MCD formulas for 21 days. (C) RNA was extracted from the livers of mice fed chow, MCS or MCD diets for 21 days. mRNA encoding XBP-1 and target genes Edem1, Erdj4 and Dnajc3 was measured by quantitative PCR (n = 5). *P < 0.05 vs. chow.