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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Gastroenterology. 2010 Jul 1;139(5):1686–1698.e6. doi: 10.1053/j.gastro.2010.06.060

Figure 1.

Figure 1

Induction of CAT2 and L-Arg uptake in RAW 264.7 macrophages and effect of spermine and L-lysine. Macrophages were incubated with H. pylori lysate (HPL) at an MOI of 100. mRNA expression for CAT1 and CAT2 was assessed at 6 h after HPL stimulation ± the inhibitor (Inhib) spermine (Spm; 12.5 μM) or L-lysine (Lys; 20 mM) by real-time PCR (A) and semi-quantitative RT-PCR (B). (C) Western blot analysis for CAT2 (80 kDa), iNOS (130 kDa) and β-actin (42 kDa) 24 h after stimulation. (D) L-Arg transport assessed as uptake of 14C-L-Arg into cells at the times indicated. (E and F) NO levels measured as concentration of NO2−, at 24 h after stimulation. In (F), L-Arg was added to L-Arg-free, serum-free medium ± Spm. In A–F, data are from 3 experiments performed in duplicate. For A and E, **P < .01 vs control; for D, *P < .05, **P < .01 vs control, HPL + Spm, and HPL + Lys; for E and F, §§P < .01, §§§P < .001 vs HPL alone.