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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Gastroenterology. 2010 Jul 1;139(5):1686–1698.e6. doi: 10.1053/j.gastro.2010.06.060

Figure 3.

Figure 3

Transfection of ODC siRNA enhances L-Arg uptake and iNOS protein expression in RAW 264.7 macrophages. (A) Real-time PCR for ODC with stimulation with lysates of C. rodentium (C. rod), C. jejuni (C. jej) and HPL all at an MOI of 100. (B) top panel, RT-PCR for CAT2; bottom panel, Western blot analysis for CAT2 and β-actin. (C) L-Arg transport (Scr, scrambled). *P < .05, **P < .01 vs control macrophages transfected with scrambled siRNA; §§P < .01 vs HPL-stimulated macrophages transfected with scrambled siRNA. (D) Western blot analysis for iNOS and β-actin. (E) Densitometry of iNOS Western blots, standardized to β-actin. (F) NO2− levels measured at 24 h after stimulation. In E and F, **P < .01; ***P < .001 vs macrophages transfected with scrambled siRNA. n = 3–4 for all experiments.