Skip to main content
. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Hepatology. 2010 Nov;52(5):1680–1689. doi: 10.1002/hep.23848

Figure 1. SULF2 increases Wnt3a expression and enhances Wnt/β-catenin signaling in HCC cells.

Figure 1

(A) Hep3B Vector and Hep3B SULF2-H cells were treated with 0, 2, and 10 ng/ml of Wnt3a ligand for 24 hours, washed and lysed, and Western immunoblotting performed using antibodies against Wnt3a and actin (loading control). SULF2 increased basal and Wnt3a-induced Wnt3a expression. (B and C) TOPFLASH luciferase assay showing the effect of SULF2 on Wnt3a-induced Tcf/Lef transcriptional activity in HCC cells. Hep3B cells were transfected with a TOPFLASH reporter construct and either SULF2-expressing construct or an empty vector. After serum-starvation, cells were treated with 5 ng/ml Wnt3a and TOPFLASH luciferase activity was measured after 6 hours (B) or 24 hours (C). SULF2 enhanced Wnt3a-induced luciferase activity as early as 6 hours and the effect was sustained over 24 hours (p<0.0002).