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. Author manuscript; available in PMC: 2011 Dec 28.
Published in final edited form as: Cancer Lett. 2010 Sep 19;299(2):137–149. doi: 10.1016/j.canlet.2010.08.012

Fig. 5.

Fig. 5

Knockdown of MMP-1 or MMP-2 “rescues” the highly invasive phenotype resulting from RKIP knockdown. (A) Expression of MMP-1 and MMP-2 in the RKIP/MMP-1 and RKIP/MMP-2 double-knockdown T47D cells, as determined by quantitative real-time RT-PCR (with glyceraldehyde-3-phosphate dehydrogenase, whose expression was unaffected by knockdown of RKIP and the MMPs, as an internal standard). “Control” refers to cells expressing a control siRNA for firefly luciferase alone. (B) Double knockdown of RKIP and MMP-1 or MMP-2 restores a normal rate of invasion of T47D cells. The values represent the means and SEM for the number of cells invading through Matrigel for nine wells from three independent experiments. “Control” refers to cells expressing a control siRNA for firefly luciferase, either alone or in combination with expression of siRNAs for MMP-1 or MMP-2, as indicated. Invasion of control and RKIP-single-knockdown cells was evaluated in experiments independent from those in Fig. 2 and in parallel with the double-knockdown cells.