Table 1.
Sensitivity of RKIP-knockdown and RKIP-overexpressing cells to pharmacological inhibition of MMPs.
| Cell type | MICa (µM) | IC50b (µM) | MLCc (µM) |
|---|---|---|---|
| BT-20 control | 0.5 | 1.6 | 20 |
| BT-20 RKIP KD | 1 | 3.2 | 20 |
| T47D control | 0.5 | 1.8 | 20 |
| T47D RKIP KD | 0.5 | 3.0 | 20 |
| LoVo control | No subtoxic activity | NA | 20 |
| LoVo RKIP KD | 1 | 5.4 | 20 |
| MDA-MB-435 control | 5 | 7.3 | 20 |
| MDA-MB-435 HA-RKIP (RKIP-overexpressing) |
No subtoxic activity | NA | 20 |
Minimum inhibitory concentration (MIC) represents the lowest concentration at which GM 1489, a general MMP inhibitor, exhibited a statistically significant antiinvasive effect by Student’s t tests for each cell type. Data are derived from three independent experiments.
The half-maximal inhibitor concentration (IC50) for inhibition of invasion by GM 1489. Only subtoxic concentrations, for which there was no sign of cytotoxicity in “titration” experiments done initially, were used for the IC50 determinations with GraphPad Prism software.
Minimum lethal concentration (MLC) represents the lowest concentration at which signs of cytototoxicity were first observed based on the trypan blue dye exclusion assay in preliminary “titration” experiments.