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. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: Eur J Pharmacol. 2010 Sep 18;649(1-3):51–58. doi: 10.1016/j.ejphar.2010.08.057

Fig. 5.

Fig. 5

Antagonistic effect of genistein and apigenin on NSC-741909-induced JNK activation and apoptosis. (A) Effect on JNK activation. After pretreatment with NSC-741909 for 1 hour, H460 cells were treated with 10 μM genistein or 10 μM apigenin for the times indicated in the text. Cells were harvested at the time when the total NSC-741909 treatment reached 12 h. Cells treated with DMSO were set as the control. We performed Western blot analysis to detect the changes of JNK phosphorylation. (B) Effect on apoptosis. H460 cells were pretreated with 10μM of genistein, apigenin or SP600125 (a JNK inhibitor) for half hour before addition of 1μM of NSC-741909. The cells were harvested 24 hours after NSC-741909 treatment. Levels of caspase 8 cleavage and cleaved PARP were determined.