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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Aug 14;1800(12):1241–1247. doi: 10.1016/j.bbagen.2010.08.001

Figure 5. Mutations V161A and F357C inhibit NHAoc/NHA2 Na+ antiport activity and fail to complement the salt-sensitive phenotype of BW31a cells.

Figure 5

(−) cells transfected with an empty vector, (+) yeast ScNha1, (WT) WT NHAoc/NHA2, I159T, V161A and F357C: three mutants of NHAoc/NHA2. A) Intracellular Na+ content after culturing BW31a cells for 30 minutes in 100 mM NaCl. B) Growth curves in YNB/0.6 M NaCl of BW31a cells expressing different mutants of NHAoc/NHA2.