. Author manuscript; available in PMC: 2011 Dec 17.

Published in final edited form as: Neurosci Lett. 2010 Sep 29;486(3):235–239. doi: 10.1016/j.neulet.2010.09.061

Table 1.

CI vs CIV activities and substrate-specific respiration in ST DA vs non DA synaptosomes isolated from 12 month old high vs low (HE vs LE) A53T α-syn expressing transgenics. Uncoupled complex I-mediated oxygen consumption was measured in isolated synaptosomes using glutamate/malate as substrate in the presence of ADP and carbonyl cyanide p-trifluoromethoxyphexylhydrazone. Rotenone-sensitive respiration was calculated as the difference between oxygen consumption rate pre- and post-rotenone addition (2 μM). KCN-sensitive complex IV-mediated respiration was similarly measured using TMPD/ascorbate as substrate. n = 3 for each condition.

	CI-mediated oxygen consumption (rotenone inhibitable, nM O₂/min)	CIV-mediated oxygen consumption (KCN inhibitable, nM O₂/min)
LE DA	7.8 +/− 0.4	33.4
HE DA	3.3 +/− 0.8^a	37.1
LE NDA	17.9+/− 0.6	34.5
HE NDA	17.7+−0.4	35.1
	CI activity (μM NADH/μg/min	CIV activity (μM Fe-Cyt/μg/min
LE DA	17.15+/− 2.81	10.16+/− 1.68
HE DA	9.92+/− 0.29^a	9.0 +/−0.72
LE NDA	20.16+/− 2.33	8.24 +/− 0.34
HE NDA	19.38+/− 1.7	8.6 +/− 1.2

p <0.05 compared with LE controls.