Organization of T.thermophila DNA
at the mse2.9 locus and construction of heh2.2. MAC, macronuclear
DNA; MIC, micronuclear DNA; ARP1, acidic repetitive
protein. The area inside the dotted lines indicates micronuclear-limited
DNA. Outside the lines macro- and micronuclear sequence are identical.
Hatched and clear boxes denote ARP1 exons and introns,
respectively. Construction of heh2.2 has been described previously
(12). The solid line beneath unprocessed heh2.2 represents the probe
used for Southern analysis. Intron boundaries that flank mse2.9
are indicated by thick vertical lines. H, HindIII;
X, XbaI; E, EcoRI.