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. 2001 Jan 15;29(2):488–498. doi: 10.1093/nar/29.2.488

Figure 4.

Figure 4

Deletion analysis reveals that mse2.9 requires 81 bp of sequence on the right side for efficient and accurate processing. (A) The thick horizontal line represents micronuclear-limited sequence of heh2.2. (B) Southern blot analysis of genomic DNA purified from transformants of heh2.2+38R (lanes 1–5), heh2.2+60R (lanes 6–9) and heh2.2+81R (lanes 10–13) clones. Whole-cell DNA was digested and probed as in Figure 2. Longer exposure for some lanes was performed to increase visualization of DNA fragments in low abundance. (C) Sequence analysis of several deletion clone transformants as in Figure 2.