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. Author manuscript; available in PMC: 2011 Apr 21.
Published in final edited form as: Oncogene. 2010 Aug 2;29(42):5678–5686. doi: 10.1038/onc.2010.308

Figure 5.

Figure 5

p27 regulation is involved in the growth-promoting effects of estrogen. a, expression analysis of p27 mRNA in the thyroid and liver from control and mutant mice. b, expression analysis of p27 mRNA in a mouse thyroid cancer cell line upon estrogen treatment. c, Western blot analysis of p27 levels in a mouse thyroid cancer cell line 24 hours after estrogen treatment. d, expression analysis of p27 mRNA in the thyroid from control and mutant males, as well as old (>1 year) males that had developed hyperplastic or neoplastic (Ad/Ca: Adenoma, Carcinoma) lesions. e, Kaplan-Meyer analysis showing the effect of progressive reduction of p27 gene dosage on the survival of mutant mice. f, Kaplan-Meyer analysis showing that 50% reduction of p27 gene dosage abolishes the gender-dependent differences in mutant survival. g, proliferative index (Ki-67 positive thyrocytes) of single and Pten−/−;p27+/− compound mutant thyroids. h, prevalence of thyroid lesions in compound mutant mice.