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. Author manuscript; available in PMC: 2010 Nov 2.
Published in final edited form as: Cell Mol Bioeng. 2008 Dec 1;1(4):229–239. doi: 10.1007/s12195-008-0028-4

FIGURE 1.

FIGURE 1

SPR reflectance variations of a cell monolayer induced by AngII activation. (a) Cells were grown on a gold-coated glass slide and placed on top of a BK7 coupling prism with a layer of refractive index matching fluid. A P-polarized laser diode is reflected on the sample and passed through a motorized linear polarizer and the intensity of the polarized laser beam is measured with a voltage-biased photodetector. (b) The SPR signal is plotted as the laser reflectance variation (RV) as a function of time (min). The baseline prior to the injection of 100 nM AngII shows a constant plateau (I), immediately followed by a rapid diminution in the RV signal after the stimulation by AngII (II). The SPR signal then increases to a level exceeding the baseline (III). (c) Control experiments showing SPR response for cells are stimulated with the vehicle (HBSS) or pretreated with an AT1 antagonist (EXP3174) and stimulated with 100 nM AngII.