Skip to main content
. Author manuscript; available in PMC: 2010 Nov 2.
Published in final edited form as: Methods Enzymol. 2009;455:95–125. doi: 10.1016/S0076-6879(08)04204-3

Figure 4. Unfolding and 1D-Ising analysis of capped consensus ankyrin repeat proteins.

Figure 4

Data are from Pluckthun et al. (Wetzel et al., 2008), and were obtained using the the program DigitizeIt 1.5 for Mac OSX (http://www.digitizeit.de). Solid lines result from fitting a heteropolymer partition function, assuming the N- and C-caps have identical intrinsic folding energies that differ from the value for the internal repeats. Likewise, the effect of guanidine is partitioned into intrinsic folding energies and is allowed to vary between the capping and internal repeats. The pre-transition for NI3C appears to partly resolve the parameters from the capping and internal repeats.