Table 1.
Free energies of folding of capped consensus repeat protein constructs.
| nrep | Construct | Folding free energy (D→N) | |
|---|---|---|---|
| A | 3 | R3 | ΔG° = 3ΔGR + 2ΔGi,i +1 |
| B | 4 | R4 | ΔG° = 4ΔGR + 3ΔGi,i +1 |
| C | 5 | R5 | ΔG° = 5ΔGR + 4ΔGi,i +1 |
| D | 3 | NRC | ΔG° = 1ΔGN + 1ΔGR + 1ΔGC + 2ΔGi,i +1 |
| E | 4 | NR2C | ΔG° = 1ΔGN + 2ΔGR + 1ΔGC + 3ΔGi,i +1 |
| F | 5 | NR3C | ΔG° = 1ΔGN + 3ΔGR + 1ΔGC + 4ΔGi,i +1 |
| G | 6 | NR4C | ΔG° = 1ΔGN + 4ΔGR + 1ΔGC + 5ΔGi,i +1 |
| H | 4 | NR3 | ΔG° = 1ΔGN + 3ΔGR + 3ΔGi,i +1 |
| I | 5 | NR4 | ΔG° = 1ΔGN + 4ΔGR + 4ΔGi,i +1 |
| J | 4 | R3C | ΔG° = 3ΔGR + 1ΔGC + 3ΔGi,i +1 |
| K | 5 | R4C | ΔG° = 4ΔGR + 1ΔGC + 4ΔGi,i +1 |
N- and C-terminal caps are assumed to differ in intrinsic folding energy from consensus repeats (R), but have the same interfacial energy (ΔGi,i+1). Relaxing this restriction would introduce two additional interfacial energy terms (N:R and R:C as well as R:R).