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. 2010 Oct;23(5):487–495. doi: 10.1089/vim.2010.0010

FIG. 2.

FIG. 2.

Blocking the PD-1 pathway inhibits SOCS-1 protein expression in healthy T cells exposed to HCV core protein. (A) SOCS-1 upregulation in healthy T cells exposed to HCV core protein. CD4+ T cells isolated from healthy donors using antibody-conjugated magnetic beads were stimulated with anti-CD3/CD28 in the presence or absence of HCV core protein for 24 h. SOCS-1 protein expression was detected by Western blot, and β-actin served as a control. (B) PD-1 blocking inhibits HCV core-induced SOCS-1 expression in activated T cells. T cells isolated from healthy donors as above were treated with anti-PD-1, anti-PDL-1, or control antibody for 6 h, followed by stimulation with anti-CD3/CD28 and HCV core protein for 24 h. SOCS-1 protein expression was detected by Western blot, and β-actin served as a control.