Figure 6.

Inhibiting Rho kinase restored eNOS expression in BAEC. Western Blot analysis of eNOS expression showed that cultures of BAEC maintained in high glucose (HG, 25 mM) or peroxynitrite (PN, 100 μM) showed significant reduction in eNOS expression (40%) compared to normal glucose (NG, 5 M). Cotreatment with the specific Rho-kinase inhibitor Y26732 (Y, 1 μM) restored eNOS expression in high glucose or peroxynitrite-maintained cultures. (n = 4) Values are expressed as means ± SEM, *P < .05 versus control.