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. 2001 Jan 15;29(2):479–487. doi: 10.1093/nar/29.2.479

Figure 2.

Figure 2

Figure 2

SRE complexes isolated from HEK 293 cell lysates. (A) Oligonucleotide sequences used in this study. Inverted arrows indicate the palindromic sequences while ets binding sites and CArG boxes are bracketed. Asterisks above the PalXE sequence indicate CArG box mutations. (B) Proteins isolated by scrambled (lanes 1 and 2), SRE (lanes 3 and 4), PalRE (lanes 5 and 6) and PalSE (lanes 7 and 8) duplexes from serum-starved (–) or EGF-treated (+) HEK 293 cells resolved by SDS–PAGE and stained with SYPRO ruby. Numbered arrows indicate the proteins analysed in this work. The asterisk between lanes 2 and 3 indicates the 80 kDa protein referred to in the text. (C) Proteins isolated by scrambled (lanes 1 and 2), PalXE (lanes 3 and 4), ScraPal (lanes 5 and 6) and ScraPalΔ (lanes 7 and 8) duplexes from serum-starved (–) or EGF-treated (+) HEK 293 cells. Labelling as in (B).