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S1 nuclease sensitivity of SRE duplexes. (A) DNA duplexes 32P-labelled at one end were incubated with S1 nuclease and the digestion products resolved on a 15% sequencing gel. Markers correspond to two oligonucleotides of 35 and 17 nt similarly end-labelled. Arrows indicate S1 cleavage within the A/T-rich CArG box. (B) Full-length duplexes before (–) and after (+) S1 digestion.
