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. 2001 Jan 15;29(2):415–422. doi: 10.1093/nar/29.2.415

Figure 1.

Figure 1

Effect of ratio of Eufectin to HYB0432 on TS expression. RKO cells were incubated with different Eufectin:HYB0432 combinations. After 6 h, complexes were removed and fresh RPMI medium was added. After an additional 24 h, cells were harvested and processed for western blot analysis as described in Materials and Methods. Lane 1 contains cell extracts from untreated RKO cells. Lanes 2–6 contain 190 nM HYB0432 complexed with Eufectin in weight ratios of 1:1 (lane 2), 1:3 (lane 3), 1:5 (lane 4), 1:7 (lane 5) and 1:9 (lane 6). Lane 7 contains cell extracts treated with Eufectin alone (10 µg/ml).