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. 2001 Jan 15;29(2):415–422. doi: 10.1093/nar/29.2.415

Figure 2.

Figure 2

Western blot analysis of RKO cells after treatment with HYB0432. Cells were incubated in the absence (lane 1) or presence (lanes 2–8) of Eufectin–ORN complexes (5:1 ratio; µg:µg) for 6 h, grown for an additional 24 h and then harvested and processed for western blot analysis as described in Materials and Methods. Extracts in lanes 2–5 were treated with complexes containing 63, 126, 190 and 250 nM HYB0432, respectively. Cells were treated with various control ORNs: lane 6, sense ORN HYB0848 (250 nM); lane 7, mismatch ORN HYB0503 (250 nM). Lane 8 contains cell extracts treated with Eufectin alone (13 µg/ml).